In collaboration with Mike Schutkowski lab (Halle, Germany) we have developed a new continuous fluorescent sirtuin activity assay using the native myristoyl residue at the lysine side chain of a substrate. Excellent kinetic parameters of new substrates allowed to reliably determine inhibition constants for enzyme concentrations as low as 50 pM using different microtiter plate formats.
Our new paper in Autophagy we uncovered an evidence that the mitochondrial oxidative phosphorylation supports autophagy and provides the stress…
In collaboration with Rommier, Schutkowski, Krammer, and Sippl groups, we contributed our expertise in protein biochemistry and heterologous expression to…
Congratulation to Zorka and all co-authors on the BBRC manuscript detailing the characterization of the suspected pathological H333R variant of…
As with a good wine, some things need time! This manuscript is such a case as it has matured for…
Congratulation to Lucia on her successful PhD defense at the Faculty of Natural Sciences, Charles University, Prague! It was a…
Warm welcome to Alina, who joined our lab as a bachelor student. Her work will focus on orthogonal expression techniques…
Together with the Bryja lab (Masaryk University, Brno) we have secured funding from the CSF for the period 2022 –…
Congratulations to Zorka on her first-author manuscript decribing heterologous expression of human protoporphyrinogen oxidase IX in eukaryotic systems and the…
As a result of our long-lasting collaboration with Mike Schutkowski group (Martin-Luther-Universität Halle-Wittenberg, Halle), we have developed a new continous…
Warm welcome to Eva, who joined our lab as a PhD student after graduating from the Faculty of Science, Charles…
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